Granny says, "Nothing like this has happened to me since the boys got back from Gettysburg!"
The Battle of Gettysburg was in 1863, 90 years before this short was made. Assuming Granny was of a marriageable age for the time, (or WAY younger than would have been considered proper), this means Granny is over 105 years old.
Granny's real name is revealed to be be Emma Webster (Corn on the Cop reveals her surname).
Sam challenges Bugs to a traditional duel, complete with a glove slap. But Bugs then mentions the Marquis of Queensbury rules, which apply to boxing matches.
Bugs imitates Pepe Le Pew here (who is modeled after Charles Boyer).
Yosemite Sam would once again pursue a wealthy widow nine years later in the short Honey's Money (1962). In that scenario, the action is complicated by the presence of the widow's obnoxious son.