Olan Soule, Casey Kasem, Larry Storch, and Ted Knight all reprise their voice roles from The Adventures of Batman (1968) as Batman, Robin, Joker and Penguin respectively, giving the guest stars authenticity in keeping the performers consistent. In addition, it also gave Kasem the unique distinction of not only performing his regular role of Shaggy, but also 'Guest Starring' in one of his already established voice roles.
This marked the first time Batman and Robin appeared in a Hanna-Barbera cartoon. Though there are subtle differences, the characters of Batman, Robin, Joker and Penguin are based upon their appearance in the (then recent) Filmation series The Adventures of Batman (1968), as well as using the familiar voice actors from that show for all four characters (including Casey Kasem as Robin who had played the role prior to being cast as Shaggy).
This is the first time the word "Jinkies" is used in the Scoobyverse, when it is spoken by Shaggy. Later, it would be adopted as Velma's catchphrase.
The unseen Riddler is named as a possible suspect. He later faced off against the same heroic alliance in Scooby-Doo & Batman: The Brave and the Bold (2018).