Fitz mentions the term "inhuman". This is the first time in the Marvel Cinematic Universe that the Inhumans are directly referenced.
The Hydra operative played by Henry Goodman who appeared with Baron Von Strucker in the mid-credit scene from Captain America: The Winter Soldier (2014) makes an appearance, and is revealed to be named Dr. List.
"Aftershocks" originally aired on ABC on March 3, 2015, and according to Nielsen Media Research, was watched by 4.48 million viewers.